Useful papers page 5

NB Please only use the downloadable resources and academic papers on this website for your own personal study and tuition.
They are not to be multiply-distributed, or exploited for commercial use.

Two-photon & Multi-photon

  1. Denk, W. & Svoboda, K. (1997) Photon Upmanship: Why Multiphoton Imaging is more than a Gimmick. Neuron 18: 351-35
  2. Helmchen, F & Denk, W (2005) Deep tissue two-photon microscopy Nature Methods 2/12: 932-940
  3. Stutzmann, GE & Parker, I (2005) Dynamic multiphoton imaging: a live view from cells to systems Physiology 20: 15-21
  4. Friedl, P et al (2007) Biological second and third harmonic generation microscopy Curr Protoc Cell Biol. Unit 4.15
  5. Campagnola, PJ & Loew, LM (2003) Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms Nature Biotech. 21/11: 1356-1360
  6. Yang, W & Yuste, R (2017) In vivo imaging of neural activity Nature Methods 14/4: 349-359
  7. Ware, LA (2014) Three photons are better than two Biophotonics 57/5: 237; 239
  8. Norris, G et al (2012) A promising new wavelength region for three-photon fluorescence microscopy of live cells for Jour. Microscopy 246/3: 266-273
  9. Yound, MD et al (2015) A pragmatic guide to multiphoton microscope design Adv. Opt. Photonics 7/2: 276-378
  10. Amor, A et al (2016) Widefield two-photon excitation without scanning: live cell microscopy with high time resolution and low photo-bleaching PLoS One 11/1: e0147115
    See also the multi-photon spectra on the Fluorophore Databases page.

TIRF – Total Internal Reflection Fluorescence

  1. Martin-Fernandez, ML; Tynan, CJ and Webb, SED (2013) A ‘pocket guide’ to total internal reflection fluorescence Jour. Microscopy 252/1: 16-22
  2. Vizcay-Barrena, G et al (2011) Subcellular and single-molecule imaging of plant fluorescent proteins using TIRFM Jour. Exp. Botany 62/15: 5419-5428
  3. Mattheyses, AL et al (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist Jour. Cell Science 123/21: 3621-3628.


  1. Sprague, BL & McNally, JG (2005) FRAP analysis of binding: proper and fitting Trends in Cell Biology 15/2: 84-91.
    See also the follow-up paper: Mueller et al (2010) Curr. Opin. Cell Biology 22/3: 403-411
  2. Day, CA et al (2012) Analysis of protein and lipid dynamics using confocal fluorescence recovery after photobleaching (FRAP) Curr Protoc Cytom. Unit 2.19
  3. Kang, M et al  (2012) Simplified Equation to Extract Diffusion Coefficients from Confocal FRAP Data Traffic 13/12: 1589–1600
  4. Mueller, F et al (2010) FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? Curr Opin Cell Biol. 22/3: 403-411
  5. Dunn, GA et al (2004) Fluorescence localization after photobleaching (FLAP) Curr Protoc Cell Biol. Unit 21.2
  6. Basics of FRET microscopy
  7. Shrestha, D et al (2016) Understanding FRET as a Research Tool for Cellular Studies Int. Jour. Mol. Studies 16: 6718-6756
  8. Bajar, BT (2016) A guide to fluorescent protein FRET pairs Sensors 16/9 pii: E1488
  9. Sarkar, P et al (2009) Photophysical properties of Cerulean and Venus Fluorescent Proteins Jour. Biomed Opt. 14/3: 034047
  10. Vogel, SS et al (2014) Estimating the distance separating fluorescent protein FRET pairs Methods 66/2: 131-138
  11. Shamirian, A et al (2015) QD-Based FRET Probes at a Glance Sensors 15/6: 13028-13051
  12. Arai, Y & Nagai, T (2013) Extensive use of FRET in biological imaging Microscopy (Oxf) 62/4: 419-428
  13. Pietraszewska-Bogiel, A & Gadella, TWJ (2010) FRET microscopy: from principle to routine technology in cell biology Jour. Microscopy 241/2: 111-118
  14. Sahoo, H (2011) Förster resonance energy transfer – A spectroscopic nanoruler: Principle and applications Jour. Photochem. Photobiol. C: Photochemistry Reviews 12/1: 20-30
  15. Piston, DW & Kramers, G-J (2007) Fluorescent protein FRET: the good, the bad and the ugly Trends Biochem. Sci. 32/9: 407-414
  16. See the Acceptor FRET and Sensitized-emisison FRET under protocols on the Edinburgh imaging facility website
  17. Chart of images required for FRET processing
NB Please only use the downloadable resources and academic papers on this website for your own personal study and tuition.
They are not to be multiply-distributed, or exploited for commercial use.

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