Wikipedia can be of great use as a good introduction to many topics, and I’ve used its links throughout these webpages. Nevertheless, please remember that the Internet is not peer-reviewed, unlike a reputable scientific journal, which is peer-reviewed. For this good reason, some people shun Wikipedia; I reckon you should use your judgement!
Microscopy Primer is a major site. It will tell you all (or nearly all) that you’ve ever wanted to know about microscopy but were afraid to ask. The style of its illustrations and java tutorials are very recognisable. Be aware that you may have to drill down to find the wood amongst the trees in this vast site. This website is supported by Nikon and Olympus. From this site you will find two useful downloadable PDFs
- Basics and Beyond by Mortimer Abramowitz. It is 20.7 Mb and 50 A4 pages.
- Optical Microscopy by Michael W. Davidson and Mortimer Abramowitz. It is 1.8Mb and 41 A4 pages. Description on Webpage.
Concepts and formulae
Many of the basic concepts in optical microscopy can be distilled into a few rules and formulae. This section is from the Nikon MicroscopyU website.
For an explanation of resolving power, and the relevant formulae, see this webpage within this site.
General Microscopy URL from Wikipedia
Principles of confocal URLs from Carl Zeiss
Zeiss image browser
Leica image browser
Olympus image viewer
Volocity Lite image browser
DeltaVision image loader for Image J
Fluorophore – Spectral viewers
Chambers on coverslips, live-cell imaging dishes
Freeware for Extended focus stacking
1. Combine ZM http://hadleyweb.pwp.blueyonder.co.uk/CZM/News.htm
Picasa image viewer – http://picasa.google.co.uk/
Fast Stone Image viewer – http://www.faststone.org/FSViewerDownload.htm
Merging Images in Photoshop
Further links on using Adobe Photoshop
1. Adobe Photoshop tutorial link
2. Adobe Photoshop for scientists tutorial page
Basic tutorials in Digital Imaging
1. http://www.dl-c.com/basics.pdf by Jonathan Sachs
6. http://www.rawlight.com/resolution.pdf by Jerry Sedgewick
Overall Microscopy Primer digital imaging index: http://micro.magnet.fsu.edu/primer/digitalimaging/index.html
More advanced, and mainly on photographic cameras
Norman Koren´s webpage – has just about everything on it
Restoring Vision (Roska & Sahel, 2018) – an inspirational paper
Vos, BE et al (2021) Designing a high-resolution LEGO-based microscope for an educational setting BioRχiv pre-print
Wikipedia can be of great use as a good introduction to many topics.
Nevertheless, please remember that the Internet is not peer-reviewed, unlike a reputable scientific journal, which is peer-reviewed.
The LEGO microscope