Useful Links

Suppliers

Download a PDF of other useful microscopy-related links.

Wikipedia can be of great use as a good introduction to many topics, and I’ve used its links throughout these webpages. Nevertheless, please remember that the Internet is not peer-reviewed, unlike a reputable scientific journal, which is peer-reviewed. For this good reason, some people shun Wikipedia; I reckon you should use your judgement!

Microscopy Primer

Microscopy Primer is a major site. It will tell you all (or nearly all) that you’ve ever wanted to know about microscopy but were afraid to ask. The style of its illustrations and java tutorials are very recognisable. Be aware that you may have to drill down to find the wood amongst the trees in this vast site. This website is supported by Nikon and Olympus. From this site you will find two useful downloadable PDFs

  1. Basics and Beyond by Mortimer Abramowitz. It is 20.7 Mb and 50 A4 pages.
  2. Optical Microscopy by Michael W. Davidson and Mortimer Abramowitz. It is 1.8Mb and 41 A4 pages. Description on Webpage.

Concepts and formulae

Many of the basic concepts in optical microscopy can be distilled into a few rules and formulae. This section is from the Nikon MicroscopyU website.

For an explanation of resolving power, and the relevant formulae, see this webpage within this site.

 

General Microscopy URL from Wikipedia

http://en.wikipedia.org/wiki/Microscopy

Principles of confocal URLs from Carl Zeiss

1. http://www.zeiss.de/C12567BE00472A5C/EmbedTitelIntern/KonfokalesPrinzipe/$File/
Confocal_Principle.pdf

2. http://www.zeiss.com/C1256D18002CC306/0/F99A7F3E8944EEE3C1256E5C0045F68B/
$file/45-0029_e.pdf

Zeiss image browser

http://www.zeiss.de/C12567BE0045ACF1/Contents-Frame/
CAA2EF638EC5F0D3C1256ADF0050E2F1

Leica image browser

1. ftp://ftp.llt.de/softlib/LCSLite/LCSLite2611537.exe

Olympus image viewer

https://support.olympus.co.jp/cf_secure/en/lisg/bio/download/ga/fv10_asw/index.cfm

Volocity Lite image browser

http://www.improvision.com/products/volocity/volocity_le/

Image J

1. http://rsb.info.nih.gov/ij/

2. http://www.macbiophotonics.ca/imagej/

DeltaVision image loader for Image J

http://www.embl.de/eamnet/html/dv_loader.html

Fluorophore – Spectral viewers

1. http://pingu.salk.edu/flow/fluo.html
2. http://www.analytchem.tugraz.at/fluorophores/
3. http://info.med.yale.edu/genetics/ward/tavi/FISHdyes2.html
4. http://www.abcam.com/ps/pdf/protocols/Fluorophore%20table.pdf
5. http://www.olympusfluoview.com/theory/fluorochromedata.html

Specimen preparation

1. http://www.seas.upenn.edu/%7Econfocal/sample_prep.html

2. http://www.uhnresearch.ca/facilities/wcif/PDF/Mountants.pdf

3. http://www.ihcworld.com/

4. http://www.ihcworld.com/

Chambers on coverslips, live-cell imaging dishes

http://www.bioptechs.com/lc_basics.html

http://www.science.mcmaster.ca/biochem/faculty/truant/protocols.htm

http://www.aecom.yu.edu/aif/instructions/coverslips/coverslips.htm

Fluorescent proteins

http://micro.magnet.fsu.edu/primer/techniques/fluorescence/
fluorescentproteins/fluorescentproteinshome.html

Freeware for Extended focus stacking

1. Combine ZM   http://hadleyweb.pwp.blueyonder.co.uk/CZM/News.htm

2. Extended focus webpage with further links

Image viewers

Picasa image viewer – http://picasa.google.co.uk/

Fast Stone Image viewer – http://www.faststone.org/FSViewerDownload.htm

Merging Images in Photoshop

1. http://www.aecom.yu.edu/aif/instructions/merging01/merging01.htm

2. http://microscopy.uc.edu/ia/PS_tutorial2.php

3. http://www.pharm.uwa.edu.au/biaf/merge.htm

Further links on using Adobe Photoshop

1. Adobe Photoshop tutorial link
http://www.adobe.com/digitalimag/science.html#resources

2. Adobe Photoshop for scientists tutorial page
http://www.rawlight.com/quickphotoshop.html

Basic tutorials in Digital Imaging

1. http://www.dl-c.com/basics.pdf by Jonathan Sachs

2. http://www.library.cornell.edu/preservation/tutorial/intro/intro-01.html

3. http://www.ischool.utexas.edu/technology/tutorials/graphics/digital/

4. http://micro.magnet.fsu.edu/primer/digitalimaging/digitalimagebasics.html

5. http://swehsc.pharmacy.arizona.edu/exppath/micro/digimageintro.html

6. http://www.rawlight.com/resolution.pdf by Jerry Sedgewick

7. http://www.chainstyle.com/tutorials/res1.html

Overall Microscopy Primer digital imaging index: http://micro.magnet.fsu.edu/primer/digitalimaging/index.html

More advanced, and mainly on photographic cameras

http://www.cambridgeincolour.com/tutorials.htm

Norman Koren´s webpage – has just about everything on it

http://www.normankoren.com/sitemap.html

Restoring Vision (Roska & Sahel, 2018) – an inspirational paper

Wikipedia can be of great use as a good introduction to many topics.

Nevertheless, please remember that the Internet is not peer-reviewed, unlike a reputable scientific journal, which is peer-reviewed.

Wikipedia main page