Understanding Light Microscopy



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Chapters

Chapter 1 Our eyes
Chapter 2 Light
Chapter 3 Basic microscope optics
Chapter 4 Microscope design
Chapter 5 Ergonomics
Chapter 6 Optical components and aberrations in the microscope
Chapter 7 The objective lens
Chapter 8 The eyepiece & condenser
Chapter 9 Illumination in the microscope
Chapter 10 Diffraction and image formation
Chapter 11 Contrast formation
Chapter 12 Reflected-light microscopy
Chapter 13 Polarised-light microscopy: theory
Chapter 14 Polarised-light microscopy: applied
Chapter 15 Fluorescence microscopy: introduction
Chapter 16 Fluorophores
Chapter 17 Optical sectioning & Confocal microscopy
Chapter 18 Operating the confocal microscope
Chapter 19 Selective-plane Illumination microscopy
Chapter 20 Bleed-through & spectral imaging
Chapter 21 Deconvolution
Chapter 22 Multi-photon microscopy
Chapter 23 TIRF microscopy
Chapter 24 FRAP & FRET
Chapter 25 Colocalisation
Chapter 26 Super-resolution techniques
Chapter 27 Fluorescence: which method? Core Facilities
Chapter 28 Biological specimen preparation
Chapter 29 Materials specimen preparation
Chapter 30 Recording the image: theory
Chapter 31 Image recording: practical aspects

Appendices

A.1 Buying, and tendering for, a light microscope
A.2 Trouble-shooting the microscope set-up
A.3 The Michel-Lévy Interference Color Chart
A.4 Cleaning & Maintenance of the microscope
A.5 Selected Manufacturer contacts
A.6 Historical overview
A.7 Microscopy timeline
Introduction
Chapters 2-3 Sample proof chapter
Table 1, Chapter 7 Objective corrections
Box 2, Chapter 7 Ernst Abbe & Numerical Aperture
Figure 21, Chapter 10 Diffraction & the image
Figure 1, Chapter 14 The polarised-light microscope
Figure 6, Chapter 14 Pleochroic behaviour under one polar
Protocol 1, Chapter 14 Setting up the polarised light microscope
Table 3, Chapter 16 Fluorescent Proteins
Box 1, Chapter 16 The Road to Gold
Box 1, Chapter 25 Colocalisation; merging red-green overlays: caveats
Table 4, Chapter 27 Collecting good images
Figure 3, Chapter 30 Digital image formation
Box 1, Chapter 31 Aliasing
Appendix 6 Historical overview